ACQUITY UPLC M-Class Trap Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.
The 2G Trap Columns are designed to minimize peak broadening while maximizing chromatographic efficiency. They can be used in a variety of 1D (low pH) nano flow applications. For 2D applications where the first dimension utilizes high pH, Waters recommends the use of 2D trap columns. This specific offering contains Waters Symmetry 100 Å pore, C18, 5 µm particles. Symmetry silica particles offer on average the highest retention for peptides and are thus most suitable for trapping applications.
The 2G V/M trap columns connect with a V-detail to the main valve and a smaller M-detail fitting to a T-piece or other suitable connection point in the LC system. Due to their design, 2G trap columns are suitable for bi-directional flow.